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Metabolic profile of the amino acids of the drinking water and earthworms (Eisenia fetida).
About 30% of the total intake of food by higher animals is excreted as “unusable” faeces. Higher plants (especially leguminous species) can recover nitrogen in the form of N-bearing amino acids. Earthworms, however, do not survive well on such sources of protein. Accordingly, most organic matter of faeces is not readily degraded by earthworms. To utilize such organic matter as food, earthworms need to extract the energy it holds in the form of C compounds. They do this by oxidizing the sugars (carbohydrates) and amino acids (protein) they find there. So they need a diversity of enzymes to hydrolyse these complex compounds into simple C and N compounds which are then absorbed and used to produce energy. Because the biomass of earthworms is closely associated with the nutritional status of the soil, they are likely to be sensitive to the concentrations of certain amino acids, sugars and other nutrients in the available pool of organic matter. Here we describe the response of Eisenia fetida, the earthworm from which biochemicals are most commonly assayed, to changes in the availability of food. We illustrate the power of manipulative techniques to determine which chemicals have the greatest influence on whole animal metabolism and key aspects of the energy budget./*
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The results of cytotoxicity studies are reported for a series of new vanadyl (V) beta-cyclodextrin (beta-CD) complexes (2a-l) prepared by reacting different V-substituted, reduced beta-CDs with aqueous V(V) solutions. The cytotoxicities of the complexes were investigated by thiazolyl blue (MTT) assay against some human cancer cell lines: DU-145 (prostate), MDA-MB-231 (breast), HOS (bone), LoVo (colon), Hep G2 (liver) and MCF-7 (breast). The study shows that the cytotoxicity of the complexes was mainly related to the complex’s V
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